Detection of bovine herpesvirus 4 DNA in cattle by realtime PCR
نویسندگان
چکیده
منابع مشابه
Detection of bovine herpesvirus 2 and bovine herpesvirus 4 DNA in trigeminal ganglia of naturally infected cattle by polymerase chain reaction.
Establishment of latent infection within specific tissues in the host is a common biological feature of the herpesviruses. In the case of bovine herpesvirus 2 (BoHV-2), latency is established in neuronal tissues, while bovine herpesvirus 4 (BoHV-4) and ovine herpesvirus 2 (OvHV-2) latent virus targets on cells of the monocytic lineage. This study was conducted in quest of BoHV-2, BoHV-4 and OvH...
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Bovine herpesvirus 1 (BoHV-1) is one of the most important infectious viruses of cattle which is considered as the causative agent of several clinical conditions in this species. Hence, we tried to isolate a local strain of the virus from carrier cattle in Ahvaz based on a strategy for reactivation of the latent infection in hosts having antibodies against the virus. Thus, 20 cattle were examin...
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We have demonstrated, by PCR and restriction enzyme analysis of the PCR product, the presence of bovine herpesvirus 4 (BoHV-4) DNA in the cell fraction of milk from dairy cattle with a history of BoHV-4 infection. We next evaluated the infectious nature of BoHV-4 DNA in those cells. Cocultivation of a BoHV-4-sensitive cell line with BoHV-4 DNA-positive milk cell samples produced cytopathic effe...
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PCR was performed on DNA extracts derived from clinical serum samples submitted for human herpesvirus 6 (HHV-6) serological examination. To detect amplified HHV-6 products, a hybridization-based microtiter plate assay (PCR ELISA; Boehringer Mannheim) was used. The assay system was found to be rapid, specific, and sensitive. Approximately three copies of a plasmid-based HHV-6 sequence could be d...
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ژورنال
عنوان ژورنال: Problems of Virology, Russian journal
سال: 2019
ISSN: 2411-2097,0507-4088
DOI: 10.36233/0507-4088-2019-64-4-178-184